Processive cleavage of long double-stranded RNAs by Dicer-2 enzyme: How are aberrant RNAs eliminated at the single-molecule level?

Masahiro Naganuma
Hisashi Tadakuma
Yukihide Tomari
Nature Communications
  • Dicer enzymes cleave aberrant long double-stranded RNAs derived from viruses etc. and produce small RNAs that suppress the expression of target genes. This phenomenon is called RNA interference or RNAi.
  • By utilizing the single-molecule imaging technology, we succeeded in directly tracing how long double-stranded RNAs are cleaved by Dicer-2 from the fruit fly, one of the most commonly used model organisms.
  • We found that Dicer-2 prefers to cleave double-stranded RNAs in a processive manner, regardless of the RNA terminal structures or the presence of its partner protein, enabling efficient elimination of aberrant RNAs.
  • This achievement advances our understanding of the molecular mechanism of RNAi and contributes to the future development of basic biological research and RNA therapeutics.


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Journal: Nature Communications

Title: Single-molecule analysis of processive double-stranded RNA cleavage by Drosophila Dicer-2
Author: Masahiro Naganuma, *Hisashi Tadakuma, *Yukihide Tomari
DOI: 10.1038/s41467-021-24555-1


Laboratory of RNA Function

Yukihide Tomari