In vitro recapitulation of the RNA silencing amplification pathway in plants : Elucidation of the mechanisms of secondary siRNA pathway important for plant differentiation and viral defense
- We present the first successful reconstitution of secondary siRNA production, a process important for plant differentiation and virus defense.
- Using the developed in vitro system, we clarified the mechanisms of how secondary siRNA production is initiated and the rules that control production efficiency.
- This work furthers our understanding of the mechanisms of plant differentiation and viral defense, and provides a mechanistic basis for the development of useful crops in the future.
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Journal: Proceedings of the National Academy of Sciences of the United States of America
Title: Cell-free reconstitution reveals the molecular mechanisms for the initiation of secondary siRNA biogenesis in plants
Author: Yuriki Sakurai, Kyungmin Baeg, Andy Y.W. Lam, Keisuke Shoji, Yukihide Tomari, Hiro-oki Iwakawa
Laboratory of RNA Function