In vitro recapitulation of the RNA silencing amplification pathway in plants : Elucidation of the mechanisms of secondary siRNA pathway important for plant differentiation and viral defense

Yuriki Sakurai
Kyungmin Baeg
Andy Y.W. Lam
Keisuke Shoji
Yukihide Tomari
Hiro-oki Iwakawa
Cell Reports
  • We present the first successful reconstitution of secondary siRNA production, a process important for plant differentiation and virus defense.
  • Using the developed in vitro system, we clarified the mechanisms of how secondary siRNA production is initiated and the rules that control production efficiency.
  • This work furthers our understanding of the mechanisms of plant differentiation and viral defense, and provides a mechanistic basis for the development of useful crops in the future.


(Go to the IQB Japanese Website)


Journal: Proceedings of the National Academy of Sciences of the United States of America

Title: Cell-free reconstitution reveals the molecular mechanisms for the initiation of secondary siRNA biogenesis in plants

Author: Yuriki Sakurai, Kyungmin Baeg, Andy Y.W. Lam, Keisuke Shoji, Yukihide Tomari, Hiro-oki Iwakawa

DOI: 10.1073/pnas.2102889118


Laboratory of RNA Function

Hiro-oki Iwakawa