日時: 令和 3 年 11月 24 日(水)17:00~18:30

開催方法: ZOOM による配信　※ 要・事前登録

講師: Puck Knipscheer, Ph.D.
Senior group leader
Genome Maintenance Laboratory
Hubrecht Institute / Oncode Institute, Utrecht, Netherlands

演題: Mechanisms that resolve replication blocking lesions, from crosslinks to G-quadruplexes

要旨:
My group focusses on deciphering the molecular details of processes that
maintain genome integrity. Specifically we are interested in replication fork
blocking lesions such as DNA interstrand crosslinks (ICLs) and G-quadruplexes.
We use a powerful biochemical system to recapitulate DNA replication and
repair under physiological conditions in vitro, based on Xenopus laevis egg
extracts.
I will focus on our latest findings regarding the resolution of mutagenic
G-quadruplex (or G4) structures. While at least 10 different DNA helicases
show G4 unwinding activity, mechanisms of how G4s are resolved in cells are
unknown. We have recently identified a comprehensive G4 unwinding mechanism
that is coupled to DNA replication and involves two helicases, DHX36 and
FANCJ. In addition, I will discuss our most recent results on another,
replication-independent, G4 resolution mechanism.

事前登録：
https://forms.office.com/r/8j4ufeVmpG

幹事: クロマチン構造機能研究分野
主催：胡桃坂ERATOクロマチンアトラスプロジェクト
共催：東京大学定量生命科学研究所
後援: 公益財団法人 応用微生物学・分子細胞生物学研究奨励会