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2010年
Narita, S., and Tokuda, H.: Biogenesis and membrane targeting of lipoproteins. In "E. coli and Salmonella", Tracy Palmer (ed), ASM Press.
Narita, S., and Tokuda, H.: Sorting of bacterial lipoproteins to the outer membrane by the Lol system. In "Methods in Molecular Biology; Protein secretion", A. Economou (ed), Humana press, in press, pp. (2010).
2009年
Nishiyama, K., and Tokuda, H.: Development of a functional in vitro integration system for an integral membrane protein, SecG. Biochem. Biophys. Res. Commun. 390, 920-924 (2009).
Tsukahara, J., Narita, S., and Tokuda, H.: Real time analysis of lipoprotein transfer from LolA to LolB by means of surface plasmon resonance. FEBS Lett., in press (2009).
Yasuda M., Iguchi-Yokoyama A., Matsuyama S., Tokuda H., and Narita S.: Membrane Topology and Functional Importance of the Periplasmic Region of ABC Transporter LolCDE. Biosci. Biotechnol. Biochem. 73, 2310-2316 (2009).
Tsukahara, J., Mukaiyama, K., Okuda, S., Narita, S., and Tokuda, H.: Dissection of the LolB function; lipoprotein binding, membrane targeting, and incorporation of lipoproteins into lipid bilayers. FEBS J. 276, 4496-4504 (2009).
Narita, S., and Tokuda, H.: Biochemical characterization of an ABC transporter LptBFGC complex required for the outer membrane sorting of lipopolysaccharides. FEBS Lett. in press doi:10.1016/j.febslet.2009.05.051. (2009).
Nakada, S., Sakakura, M., Takahashi, H., Okuda, S., Tokuda, H., and Shimada, I.: Structural investigation of the interaction between LolA and LolB using NMR. J. Biol. Chem. in press doi:10.1074/jbc.M109.001149 (2009).
Tokuda, H.: Biogenesis of outer membranes in Gram-negative bacteria. Biosci.Biotechnol. Biochem. 73, 465-473 (2009).
Okuda, S., and Tokuda, H.: Model of mouth-to-mouth transfer of bacterial lipoproteins through inner membrane LolC, periplasmic LolA, and outermembrane LolB. Proc. Natl. Acad. Sci., USA 106, 5877-5882 (2009).
2008年
Oguchi, Y., Takeda, K., Watanabe, S., Yokota, N., Miki, K., and Tokuda, H.: Opening and closing of the hydrophobic cavity of LolA coupled to lipoprotein binding and release. J. Biol. Chem. 283, 25414-25420 (2008).
Watanabe, S., Oguchi, Y., Takeda, K., Miki, K., and Tokuda, H.: Introduction of a lethal redox switch that controls the opening and closing of the hydrophobic cavity in LolA. J. Biol. Chem. 283, 25421-25427 (2008).
Kawashima, Y., Miyazaki, E., Muller, M., Tokuda, H., and Nishiyama, K.: Diacylglycerol specifically blocks spontaneous integration of membrane proteins and allows detection of a factor-assisted integration. J. Biol.Chem. 283, 24489-24496 (2008).
Okuda, S., Watanabe, S., and Tokuda, H.: A short helix in the C-terminalregion of LolA is important for the specific membrane localization of lipoproteins. FEBS Lett. 582, 2247-2251 (2008).
Antonoaea, R., Furst, M., Nishiyama, K., and Muller, M.: The periplasmic chaperone PpiD interacts with secretory proteins exiting from the SecYEG translocon. Biochemistry 47, 5649-5656 (2008).
Taniguchi, N., and Tokuda, H. : Molecular Events Involved in a Single Cycle
of Ligand Transfer from an ATP Binding Cassette Transporter, LolCDE, to a Molecular Chaperone, LolA. J. Biol. Chem. 283, 8538-8544 (2008).
2007年
Watanabe, S., Oguchi, Y., Yokota, N., and Tokuda, H. : Large-scale preparation of the homogeneous LolA-lipoprotein complex and efficient in vitro transfer of lipoproteins to the outer membrane in a LolB-dependent manner. Protein Sci. 16, 2741-2749 (2007).
Sugai, R., Takemae, K., Tokuda, H., and Nishiyama, K. : Topology inversion of SecG is essential for the cytosolic SecA-dependent stimulation of protein translocation. J. Biol. Chem. 282, 29540-29548 (2007).
Nakada, S., Sakakura, M., Takahashi, H., Tokuda, H., and Shimada, I. : Backbone resonance assignment for the outer membrane lipoprotein receptor LolB from Escherichia coli. Biomol. NMR Assign. 1, 121-123 (2007).
Hirano, Y., Hossain, Md. M., Takeda, K., Tokuda, H., and Miki, K. : Structural studies of the Cpx pathway activator NlpE on the outer membrane of Escherichia coli. Structure 15, 963-976 (2007).
Narita, S., and Tokuda, H. : The function of the ABC transporter LolCDE in protein transport to the outer membrane of E. coli. in The Enzymes, 25, 151-174 (2007).
Kanamaru, K., Taniguchi, N., Miyamoto, S., Narita, S., and Tokuda, H. : Complete reconstitution of an ATP-binding cassette transporter LolCDE complex from separately isolated subunits. FEBS J. 274, 3034-3043 (2007).
Miyamoto, S., and Tokuda, H. : Diverse effects of phospholipids on lipoprotei sorting and ATP hydrolysis by the ABC transporter LolCDE complex. Biochim. Biophys. Acta. 1768, 1848-1854 (2007).
Tanaka, S., Narita, S., and Tokuda, H. : Characterization of the Pseudomonas aeruginosa Lol system as a lipoprotein sorting mechanism. J. Biol. Chem. 282, 13379-13384 (2007).
Narita, S., and Tokuda, H. : Amino acids at positions 3 and 4 determine the membrane specificity of Pseudomonas aeruginosa lipoproteins. J. Biol. Chem. 282, 13372-13378 (2007).
Ito, H., Ura, A., Oyamada, Y., Yoshida, H., Yamagishi, J., Narita, S., Matsuyama, S., and Tokuda, H. : A new screening method to identify inhibitors of Lol (localization of lipoproteins) system, a novel antibacterial target. Microbiol. Immunol. 51, 263-270 (2007).
Tokuda, H., Matsuyama, S., and Tanaka-Masuda, K. : Structure, function and transport of lipoproteins in Escherichia coli. In The Periplasm, M. Ehrmann (ed), ASM press, 67-79 (2007).
2006年
Hirano, Y., Hossain, Md. M., Takeda, K., Tokuda, H., and Miki, K. : Purification, crystallization and preliminary X-ray crystallographic analysis of the outer membrane lipoprotein NlpE from Escherichia coli. Acta Cryst. F62, 1227-1230 (2006).
Nishiyama, K., Ikegami, A., Moser, M., Schiltz, E., Tokuda, H., and Muller, M. : A derivative of lipid A is involved in Signal Recognition Particle/SecYEG-dependent and -independent membrane integarations. J. Biol. Chem. 281, 35667-35676 (2006).
Ito, Y., Kanamaru, K., Taniguchi, N., Miyamoto, S., and Tokuda, H. : A novel ligand bound ABC transporter, LolCDE, provides insights into the molecular mechanisms underlying membrane detachment of bacterial lipoproteins. Mol Microbiol 62, 1064-1075 (2006).
渡辺祥司、徳田元：【総説】大腸菌リポタンパク質局在化の分子機構．化学と生物 44, 252〜260 (2006).
Ito, Y., Matsuzawa, H., Matsuyama, S., Narita, S., and Tokuda, H. : Genetic analysis of the mode of interplay between an ATPase subunit and membrane subunits of lipoprotein-releasing ABC transporter LolCDE. J. Bacteriol. 188, 2856-2864 (2006).
Watanabe, S., Matsuyama, S., and Tokuda, H. : Roles of the hydrophobic cavity and lid of LolA in the lipoprotein transfer reaction in Escherichia coli. J. Biol. Chem. 281, 3335-3342 (2006).
Narita, S., and Tokuda, H. : (Invited review) An ABC transporter mediating the membrane detachment of bacterial lipoproteins depending on their sorting signals. FEBS Lett. 580, 1164-1170 (2006).
2005年
成田新一郎、徳田元：【総説】大腸菌リポタンパク質の輸送．実験医学 23, 2283-2288 (2005).
Taniguchi N., Matsuyama, S., and Tokuda, H. : Mechanisms underlying energy-independent transfer of lipoproteins from LolA to LolB, which have similar unclosed β-barrel structures. J. Biol. Chem. 280, 34481-34488 (2005).
Ikegami, A., Nishiyama, K., Matsuyama, S., and Tokuda, H. : Disruption of rpmJ encoding ribosomal protein L36 decreases the expression of secY upstream of the spc operon and inhibits protein translocation in Escherichia coli. Biosci. Biotechnol. Biochem. 69, 1595-1602 (2005).
Nishiyama, K., and Tokuda, H. : Genes coding for SecG and Leu2-tRNA form an operon to give an unusual RNA comprising mRNA and a tRNA precursor. Biochim. Biophys. Acta. 1729, 166-173 (2005).
Kuruma, Y., Nishiyama, K., Shimizu, Y., Muller, M., and Ueda, T. : Development of a minimal cell-free translation system for the synthesis of presecretory and integral membrane proteins. Biotechnol. Prog. 21, 1243-1251 (2005).
2004年
Wada, R., Matsuyama, S., and Tokuda, H. : Targeted mutagenesis of five conserved tryptophan residues of LolB involved in membrane localization of Escherichia coli lipoproteins. Biochim. Biophys. Ress. Commun. 323, 1069-1074 (2004).
Tokuda, H., and Matsuyama, S. : (Invited review) Sorting of lipoproteins to the outer membrane in E. coli. Biochim. Biophys. Acta. 1693, 5-13 (2004).
Narita, S., Matsuyama, S., and Tokuda, H. : (Invited review) Lipoprotein trafficking in Escherichia coli. Arch. Micorbiol. 182, 1-6 (2004).
Sugai, R., Shimizu, H., Nishiyama, K. and Tokuda, H. : Overexpression of gnsA, a multicopy suppressor of the secG null mutation, increases acidic phospholipid contents by inhibiting phosphatidylethanolamine synthesis at low temperatures. J. Bacteriol. 186, 5968-5971 (2004).
Miyadai, H., Tanaka-Masuda, K., Matsuyama, S., and Tokuda, H. : Effects of lipoprotein overproduction on the induction of DegP (HtrA) involved in quality control in the Escherichia coli periplasm. J. Biol. Chem. 279, 39807-39813 (2004).
徳田元 : 蛋白質輸送とエンベロープの形成（原核細胞）．蛋白質・核酸・酵素49, 942-949 (2004).
西山賢一 : 大腸菌の蛋白質膜挿入におけるYidCの機能．蛋白質・核酸・酵素49, 959-960 (2004).
金丸京子、松山伸一、徳田元 : リポ蛋白質の選択的局在化機構．蛋白質・核酸・酵素49, 961-964 (2004).
松山伸一、徳田元 : 外膜蛋白質の輸送．蛋白質・核酸・酵素49, 965-966 (2004).
2003年
Hara, T., Matsuyama, S., Tokuda, H. : Mechanism underlying the inner membrane retention of E.coli lipoproteins caused by Lol avoidance signals. J. Biol. Chem. 278, 40408-40414 (2003).
Kato, Y., Nishiyama, K., and Tokuda H. : Depletion of SecDF-YajC causes a decrease in the level of SecG: Implication for their functional interaction. FEBS Lett. 550, 114-118 (2003).
Takeda, K., Miyatake, H., Yokota, N., Matsuyama, S., Tokuda, H., and Miki, K. : Crystal Structures of Bacterial Lipoprotein Localization Factors, LolA and LolB. EMBO J. 22, 3199-3209 (2003).
Takeda, K., Miyatake, H., Yokota, N., Matsuyama, S., Tokuda, H., and Miki, K. : A practical phasing procedure of MAD method without the aid of XAFS measurements: successful solution in the structure determination of the outer membrane lipoprotein carrier LolA. Acta Cryst. D59, 1440-1446 (2003).
Takeda, K., Miyatake, H., Yokota, N., Matsuyama, S., Tokuda, H., and Miki, K., : Crystallization and preliminary crystallographic study of the outer membrane lipoprotein receptor LolB, a member of the lipoprotein localization factors. Acta Cryst. D59, 1224-1226 (2003).
Narita, S., Kanamaru, K., Matsuyama, S. and Tokuda, H. : A mutation in the membrane subunit of an ABC transporter LolCDE complex causing outer membrane localization of lipoproteins against their inner membrane-specific signals. Mol. Microbiol. 49, 167-177 (2003).
松山伸一、徳田 元 : 大腸菌リポタンパク質の選択的膜局在化機構．実験医学 21, 874-879 (2003).
徳田元：【総説】細菌におけるタンパク質の局在化機構．生化学75, 109-118 (2003).
2002年
Fukuda, A., Matsuyama, S., Hara, T., Nakayama, J., Nagasawa, H. and Tokuda, H. : Aminoacylation of the N-terminal cysteine is essential for lol-dependent release of lipoproteins from membranes but does not depend on lipoprotein sorting signals. J. Biol. Chem. 277, 43512-43518 (2002).
Miyamoto, A., Matsuyama, S. and Tokuda, H. : Dominant negative mutant of a lipoprotein-specific molecular chaperone, LolA, tightly associates with LolCDE. FEBS Lett. 528, 193-196 (2002).
Nagamori, S., Nishiyama, K. and Tokuda, H. : Membrane topology inversion of SecG detected by labeling with a membrane-impermeable sulfhydryl reagent that causes a close association of SecG with SecA. J. Biochem. 132, 629-634 (2002).
Masuda, K., Matsuyama, S. and Tokuda, H. : Elucidation of the function of lipoprotein-sorting signals that determine membrane localization. PNAS 99, 7390-7395 (2002).
Narita, S., Tanaka, K., Matsuyama, S. and Tokuda, H. : Disruption of lolCDE, encoding an ATP-binding cassette transporter, is lethal for Escherichia coli and prevents the release of lipoproteins from the inner membrane. J. Bacteriol. 184, 1417-1422 (2002).
2001年
Terada, M., Kuroda, T., Matsuyama, S. and Tokuda, H. : Lipoprotein sorting signals evaluated as the LolA-dependent release of lipoproteins from the cytoplasmic membrane of Escherichia coli. J. Biol. Chem. 276, 47690-47694 (2001).
Miyamoto, A., Matsuyama, S. and Tokuda, H. : Mutant of LolA, a lipoprotein-specific molecular chaperone of Escherichia coli, defective in transfer of lipoproteins to LolB. Biochemical and Biophysical Research Communications 287, 1125-1128 (2001).
Sugai, R., Shimizu, H., Nishiyama, K. and Tokuda, H. : Overexpression of yccL (gnsA) and ydfY (gnsB) increases unsaturated fatty acids, and suppresses both the temperature-sensitive fabA6 mutation and cold-sensitive secG null mutation of Escherichia coli. J. Bacteriol. 183, 5523-5528 (2001).
Tanaka, K., Matsuyama, S. and Tokuda, H. : Deletion of lolB encoding an outer membrane lipoprotein, is lethal for Escherichia coli and causes accumulation of lipoprotein localization intermediates in the periplasm. J. Bacteriol. 183, 6538-6542 (2001).
徳田元、松山伸一：【Review】脂質で修飾された蛋白質を膜から遊離させるABCトランスポーター．蛋白質・核酸・酵素46, 1221-1227 (2001).
2000年
西山賢一：【総説】大腸菌における分泌タンパク質の膜透過分子機構 −膜透過を駆動するSecA-SecGの構造変化−．生化学72, 1383-1397 (2000).
Nishiyama, K., Suzuki, H. and Tokuda, H. : Role of the non-essential region encompassing the N-terminal two transmembrane stretches of Escherichia coli SecE. B. B. Biochem. 64, 2121-2127 (2000).
薬師寿治、松山伸一、徳田元：【総説】グラム陰性細菌におけるリポ蛋白質の生合成機構 −リポ蛋白質の選別と外膜局在化機構を中心に−．日本細菌学雑誌55, 517-526 (2000)
Nagamori, S., Nishiyama, K. and Tokuda, H. : Two SecG molecules present in a single protein translocation machinery are functional even after crosslinking. J. Biochem. 128, 129-137 (2000).
Yakushi, T., Masuda, K., Narita, S., Matsuyama, S. and Tokuda, H. : A new ABC transporter mediating the detachment of lipid-modified proteins from membranes. Nature Cell Biol. 2, 212-218 (2000).
2000年以前
Yokota, N., Kuroda, T., Matsuyama, S. and Tokuda, H. : Characterization of the LolA-LolB system as the general lipoprotein localixation mechanism of Escherichia coli. J. Biol. Chem. 274, 30995-30999 (1999).
Suzuki, H., Nishiyama, K. and Tokuda, H. : Increases in acidic phospholipid contents specifically restore protein translocation in a cold-sensitive secA or secG null mutant. J. Biol. Chem. 274, 31020-31024 (1999).
Nishiyama, K., Fukuda, A., Morita, K. and Tokuda, H. : Membrane-deinsertion of SecA underlying proton motive force-dependent stimulation of protein translocation. EMBO J. 18, 1049-1058 (1999).
Yakushi, T., Yokota, N., Matsuyama, S. and Tokuda, H. : LolA-dependent release of a lipid-modified protein from the inner membrane of Escherichia coli requires nucleoside triphosphate. J. Biol. Chem. 273, 32576-32581 (1998).
Tajima T, Yokota N, Matsuyama S, Tokuda H. : Genetic analyses of the in vivo function of LolA, a periplasmic chaperone involved in the outer membrane localization of Escherichia coli lipoproteins. FEBS Lett. 439, 51-54 (1998).
Yakushi T, Tajima T, Matsuyama S, Tokuda H. : Lethality of the covalent linkage between mislocalized major outer membrane lipoprotein and the peptidoglycan of Escherichia coli. J Bacteriol. 179, 2857-2862 (1997).
Matsuyama, S., Yokota, N. and Tokuda, H.: A novel outer membrane lipoprotein, LolB (HemM), involved in the LolA (p20)-dependent localization of lipoproteins to the outer membrane of Escherichia coli. EMBO J. 16, 6947-6955 (1997).
西山賢一、徳田元： 構造変化を繰り返す分泌蛋白質膜透過装置 蛋白質,核酸,酵素42, 1-11 (1997).
Nishiyama, K., Suzuki, T. and Tokuda, H.: Inversion of the membrane topology of SecG coupled with SecA-dependent preprotein translocation. Cell 85, 71-81 (1996).
Matsuyama, S., Tajima, T. and Tokuda, H.: A novel periplasmic carrier protein involved in the sorting and transport of Escherichia coli lipoproteins destined for the outer membrane. EMBO J. 14, 3365-3372 (1995).
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