Publication

2024

Shunsuke Sumi, Michiaki Hamada, Hirohide Saito.: Deep generative design of RNA family sequences, Nat Methods. 2024 Mar;21(3):435-443. doi: 10.1038/s41592-023-02148-8. Epub 2024 Jan 18.

Hirosawa M, Saito H.: RNA Switches Using Cas Proteins, Methods Mol Biol. 2024:2774:177-192. doi: 10.1007/978-1-0716-3718-0_12.

Si Jing Chen, Kazuya Hashimoto, Kosuke Fujio, Karin Hayashi, Sudip Kumar Paul, Akinori Yuzuriha, Wei-Yin Qiu, Emiri Nakamura, Maria Alejandra Kanashiro, Mio Kabata, Sou Nakamura, Naoshi Sugimoto, Atsushi Kaneda, Takuya Yamamoto, Hirohide Saito, Naoya Takayama & Koji Eto.: A let-7 microRNA-RALB axis links the immune properties of iPSC-derived megakaryocytes with platelet producibility, Nat Commun. 2024 Mar 22;15(1):2588. doi: 10.1038/s41467-024-46605-0.

2023

Kawasaki S*, Ono H, Hirosawa M, Kuwabara T., Sumi S., Lee S, Woltjen K., and Saito H*.: Programmable
mammalian translational modulators by CRISPR-associated proteins, Nat Commun.2023 Apr 19;14(1):2243. doi: 10.1038/s41467-023-37540-7.

Ohno H*, Akamine S, Mochizuki M, Hayashi K, Akichika S, Suzuki T, and Saito H*.: Versatile strategy using vaccinia virus-capping enzyme to synthesize functional 5′ cap-modified mRNAs, Nucleic Acids Res. 2023 Apr 11;51(6):e34. doi: 10.1093/nar/gkad019. 

Kameda S, Ohno H, and Saito H*.: Synthetic  circular  RNA  switches  and  circuits  that  control  protein expression in mammalian cells, Nucleic Acids Res. 2023 Feb 28;51(4):e24. doi: 10.1093/nar/gkac1252.

Hirohisa Ohno, Sae Akamine, Megumi Mochizuki, Karin Hayashi, Shinichiro Akichika, Tsutomu Suzuki, Hirohide Saito.: Versatile strategy using vaccinia virus-capping enzyme to synthesize functional 5′ cap-modified mRNAs, Nucleic Acids Res. 2023 Apr 11;51(6):e34. doi: 10.1093/nar/gkad019.

Shunsuke Kawasaki, Hiroki Ono, Moe Hirosawa, Takeru Kuwabara, Shunsuke Sumi, Suji Lee, Knut Woltjen, Hirohide Saito.: Programmable mammalian translational modulators by CRISPR-associated proteins, Nat Commun. 2023 Apr 19;14(1):2243. doi: 10.1038/s41467-023-37540-7.

Hiroki Ono and Hirohide Saito.: Sensing intracellular signatures with synthetic mRNAs, RNA Biol. 2023 Jan;20(1):588-602. doi: 　10.1080/15476286.2023.2244791.

Shodai Komatsu, Hirohisa Ohno, Hirohide Saito.: Target-dependent RNA polymerase as universal platform for gene expression control in response to intracellular molecules, Nat Commun. 2023 Nov 17;14(1):7256. doi: 10.1038/s41467-023-42802-5.

2022

Fujita Y, Hirosawa M, Hayashi K, Hatani T, Yoshida Y, Yamamoto T, Saito H.: A versatile and robust cell purification system with an RNA-only circuit composed of microRNA-responsive ON and OFF switches, Sci Adv. 2022 Jan 7;8(1):eabj1793. doi: 10.1126/sciadv.abj1793. Epub 2022 Jan 5.

Nakanishi H, Saito H, Itaka K.: Versatile Design of Intracellular Protein-Responsive Translational Regulation System for Synthetic mRNA, ACS Synth Biol. 2022 Mar 18;11(3):1077-1085.doi:10.1021/acssynbio.1c00567. Epub 2022 Feb 21.

Pardi ML, Wu J, Kawasaki S, Saito H.: Synthetic RNA-based post-transcriptional expression control methods and genetic circuits, Adv Drug Deliv Rev. 2022 May:184:114196. doi: 10.1016/j.addr.2022.114196. Epub 2022 Mar 11.

Iwasaki M, Kawahara Y, Okubo C, Yamakawa T, Nakamura M, Tabata T, Nishi Y, Narita M, Ohta A, Saito H, Yamamoto T, Nakagawa M, Yamanaka S, Takahashi K.: Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells, iScience. 2022 Apr 25;25(5):104289. doi: 10.1016/j.isci.2022.104289. eCollection 2022 May 20.

Tsujisaka Y, Hatani T, Okubo C, Ito R, Kimura A, Narita M, Chonabayashi K, Funakoshi S, Lucena-Cacace A, Toyoda T, Osafune K, Kimura T, Saito H*, Yoshida Y*.: Purification of human iPSC-derived cells at large scale using microRNA switch and magnetic-activated cell sorting, Stem Cell Reports. 2022 Jul 12;17(7):1772-1785. doi: 10.1016/j.stemcr.2022.05.003. Epub 2022 Jun 9.

Nakanishi H*, Yoshii T, Tsukiji S, Saito H*.: A protocol to construct RNA-protein devices for photochemical translational regulation of synthetic mRNAs in mammalian cells, STAR Protoc. 2022 Jun 10;3(2):101451. doi: 10.1016/j.xpro.2022.101451. eCollection 2022 Jun 17.

Saito H*.: The RNA world hypothesis, Nat Rev Mol Cell Biol. 2022 Sep;23(9):582. doi: 10.1038/s41580-022-00514-6.

Miyashita E, Komatsu KR, Saito H*.: Large-Scale Analysis of RNA-Protein Interactions for Functional RNA Motif Discovery Using FOREST, Methods Mol Biol. 2022:2509:279-290. doi: 10.1007/978-1-0716-2380-0_17.

Fujita Y, Kameda T, Singh CR, Pepper W, Cecil A, Hilgers M, Thornton M, Asano I, Moravek C, Togashi Y*, Saito H*, Asano K*.: Translational recoding by chemical modification of non-AUG start codon ribonucleotide bases, Sci Adv. 2022 Apr 8;8(14):eabm8501. doi: 10.1126/sciadv.abm8501. Epub 2022 Apr 8.

Ohno H, Sawanobori C, Saito H.: RNA Nanotechnology, Molecular Robotics: An Introduction

Ohno H, Komatsu S, Saito H.: Cell-fate control by RNA/RNP, Molecular Robotics: An Introduction

Rust R, Weber RZ, Generali M, Kehl D, Bodenmann C, Uhr D, Wanner D, Zürcher KJ, Saito H, Hoerstrup SP, Nitsch RM, Tackenberg C.: Xeno-free induced pluripotent stem cell-derived neural progenitor cells for in vivo applications, J Transl Med. 2022 Sep 16;20(1):421. doi: 10.1186/s12967-022-03610-5.

2021

Shibata. T., Suzuki. Y., Sugiyama. H., Endo. M., Saito. H.: Folding RNA–Protein Complex into Designed Nanostructures, Methods Mol Biol. 2021:2323:221-232. doi: 10.1007/978-1-0716-1499-0_16. 

Akagi J, Yamada T, Hidaka K, Fujita Y, Saito H, Sugiyama H, Endo M, Matsumura S, Ikawa. Y.: An RNA Triangle with Six Ribozyme Units Can Promote a Trans-Splicing Reaction through Trimerization of Unit Ribozyme Dimers, Appl. Sci. 2021 Mar 14, 11(6), 2583. doi: 10.3390/app11062583

Nakanishi H, Yoshii T, Kawasaki S, Hayashi K, Tsutsui K, Oki C, Tsukiji S, Saito H.: Light-controllable RNA-protein devices for translational regulation of synthetic mRNAs in mammalian cells., Cell Chem Biol. 2021 May 20;28(5):662-674.e5. doi: 10.1016/j.chembiol.2021.01.002. Epub 2021 Jan 27.

Minegishi K, Rothé B, Komatsu K R, Ono H, Ikawa Y, Nishimura H, Katoh T A, Kajikawa E, Sai X, Miyashita E, Takaoka K, Bando K, Kiyonari H, Yamamoto T,  Saito H, Constam D B, Hamada H.: Fluid flow-induced left-right asymmetric decay of Dand5 mRNA in the mouse embryo requires a Bicc1-Ccr4 RNA degradation complex, Nat Commun. 2021 Jul 1;12(1):4071. doi: 10.1038/s41467-021-24295-2.

Kawasaki S, Ono H, Hirosawa M, Kuwabara T, Saito H.: Programmable mammalian translational modulators by CRISPR-associated proteins, bioarxiv. 2021 Sep 17, doi: 10.1101/2021.09.17.460758

Hirosawa M, Saito H.: Cell-Type-Specific CRISPR-Cas9 System with miRNAs, CRISPR-Cas Methods. 2021 July 31;pp265–279.

Miki K, Saito H, Yoshida Y.: Isolation of Cardiomyocytes Derived from Human Pluripotent Stem Cells Using miRNA Switches, Methods Mol Biol. 2021:2320:35-51. doi: 10.1007/978-1-0716-1484-6_5.

Nakanishi H, Saito H.: Purification of Specific Cell Populations Differentiated from Stem Cells Using MicroRNA-Responsive Synthetic Messenger RNAs., Methods Mol Biol. 2021:2312:73-86. doi: 10.1007/978-1-0716-1441-9_5.

2020

Kawasaki S, Ono H, Hirosawa M, Saito H.: RNA and protein-based nanodevices for mammalian post-transcriptional circuits., Curr Opin Biotechnol. 2020 Jun:63:99-110. doi: 10.1016/j.copbio.2019.11.019. Epub 2020 Jan 11.

Parr CJC, Wada S, Kotake K, Kameda S, Matsuura S, Sakashita S, Park S, Sugiyama H, Kuang Y, Saito H.: N1-Methylpseudouridine substitution enhances the performance of synthetic mRNA switches in cells., Nucleic Acids Res. 2020 Apr 6;48(6):e35. doi: 10.1093/nar/gkaa070.

Ohno H, Akamine S, Saito H.: Synthetic mRNA-based systems in mammalian cells, Adv Biosyst. 2020 May;4(5):e1900247. doi: 10.1002/adbi.201900247. Epub 2020 Mar 2.

Nakanishi H, Saito H.: Caliciviral protein-based artificial translational activator for mammalian gene circuits with RNA-only delivery, Nat Commun. 2020 Mar 10;11(1):1297. doi: 10.1038/s41467-020-15061-x.

Komatsu KR, Taya T, Matsumoto S, Miyashita E, Kashida S, Saito H.: RNA structure-wide discovery of functional interactions with multiplexed RNA motif library., Nat Commun. 2020 Dec 8;11(1):6275. doi: 10.1038/s41467-020-19699-5.

2019

Ohno H, Akamine S, Saito H: RNA nanostructures and scaffolds for biotechnology applications., Curr Opin Biotechnol. 2019 Aug:58:53-61. doi: 10.1016/j.copbio.2018.11.006. Epub 2018 Nov 28.

Nakanishi H, Saito H.: Endogenous Signal-Responsive Transgene Switch Systems for Visualization and Purification of Specific Cells, Medical Applications of iPS Cells ( Current Human Cell Research and Applications book series), 99-112, (2019)

Nakanishi H, Saito H.: Mammalian gene circuits with biomolecule-responsive RNA devices, Curr Opin Chem Biol. 2019 Oct:52:16-22. doi: 10.1016/j.cbpa.2019.04.013. Epub 2019 May 23.

Kashida S, Wang DO, Saito H, Gueroui Z.: Nanoparticle-based local translation reveals mRNA as a translation-coupled scaffold with anchoring function., Proc Natl Acad Sci U S A. 2019 Jul 2;116(27):13346-13351. doi: 10.1073/pnas.1900310116. Epub 2019 Jun 19.

Hirosawa M, Fujita Y, Saito H.: Cell-Type-Specific CRISPR Activation with MicroRNA-Responsive AcrllA4 Switch., ACS Synth. Biol. 2019, 8, 7, 1575–1582,Publication Date:July 3,2019.https://doi.org/10.1021/acssynbio.9b00073

Ohno H, Akamine S, Saito H.: RNA nanostructures and scaffolds for biotechnology applications., Curr Opin Biotechnol. 2019 Aug:58:53-61. doi: 10.1016/j.copbio.2018.11.006. Epub 2018 Nov 28.

Saito H, Yokobayashi Y.: Editorial overview: Mammalian synthetic biology: from devices to multicellular systems., Curr Opin Chem Biol. 2019 Oct:52:A1-A2. doi: 10.1016/j.cbpa.2019.07.010. Epub 2019 Aug 26.(2019)

Endo K, Hayashi K, Saito H.: Numerical operations in living cells by programmable RNA devices., Sci Adv. 2019 Aug 21;5(8):eaax0835. doi: 10.1126/sciadv.aax0835. eCollection 2019 Aug.

Sunohara T, Morizane A, Matsuura S, Miyamoto S, Saito H, Takahashi J.: MicroRNA-Based Separation of Cortico-Fugal Projection Neuron-Like Cells Derived From Embryonic Stem Cells, Front Neurosci. 2019 Oct 23:13:1141. doi: 10.3389/fnins.2019.01141. eCollection 2019.

Ono H, Kawasaki S, Saito H.: Orthogonal Protein-Responsive mRNA Switches for Mammalian Synthetic Biology., ACS Synth Biol. 2020 Jan 17;9(1):169-174. doi: 10.1021/acssynbio.9b00343. Epub 2019 Dec 13.

2018

Yamada GT, Hori Y, Suzuki S, Kawasaki S, Hiroi N, Saito H, Funahashi A.: Mathematical modeling identifies the conditions required for RNA- based oscillator, SICE 2018 Annual Conference, Position Paper

Parr, C; Kuang, BY; Saito, H; Shin, J: Increased switch dynamics of in vitro synthesized mRNA using modified rNTP nucleosides, HUMAN GENOMICS, Volume: 12(2018)

Kashida, Shunnichi, and Hirohide Saito: Design of Ligand‐Controlled Genetic Switches Based on RNA Interference., Synthetic Biology: Parts, Devices and Applications, 8-(2018)

Endo, Kei, and Hirohide Saito.: mRNA Engineering for the Control of Mammalian Cells in Medical Applications., Applied RNA Bioscience. Springer. Singapore, 95-114(2018)

Hatani. T, Chonabayashi. K, Fujita. Y, Kimura. T, Saito. H, Yoshida. Y: Efficient Purification of Human iPSC-Derived Cardiomyocytes in a Large Scale Using a Synthetic microRNA Switch and Magnetic-Activated Cell Sorting, Circulation. 2018;138:A17344(2018)

Zhitnyuk Y, Gee P, Lung MSY, Sasakawa N, Xu H, Saito H, Hotta A: Efficient mRNA delivery system utilizing chimeric VSVG-L7Ae virus-like particles., Biochem Biophys Res Commun. 2018 Nov 10;505(4):1097-1102. doi: 10.1016/j.bbrc.2018.09.113. Epub 2018 Oct 10.

Matsuura S, Ono H, Kawasaki S, Kuang Y, Fujita Y, Saito H.: Synthetic RNA-based logic computation in mammalian cells., Nature Communications volume 9, Article number: 4847 (2018)

Zhitnyuk. Y, Gee. P, Lung. MSY, Sasakawa. N, Xu. N, Saito. H, Hotta. A: Membrane-derived virus-like particles as a transport vehicle for mRNA molecules, JOURNAL OF BIOENERGETICS AND BIOMEMBRANES, 50, 6, 602-602(2018)

Hatani. T, Okubo. C, Fujita. Y, Chonabayashi. K, Kimura. T, Saito. H, Yoshida. Y: Efficient Large-Scale Purification of Human iPSC-Derived Cardiomyocytes by Synthetic microRNA Switch and Magnetic-Activated Cell Sorting, Circulation. 2018;138:A17344:5 Nov 2018https://doi.org/10.1161/circ.138.suppl_1.173448

2017

Nakanishi H, Miki K, Komatsu KR, Umeda M, Mochizuki M, Inagaki A, Yoshida Y, Saito H.: Monitoring and visualizing microRNA dynamics during live cell differentiation using microRNA-responsive non-viral reporter vectors., Biomaterials. 2017 Jun:128:121-135. doi: 10.1016/j.biomaterials.2017.02.033. Epub 2017 Mar 2.

Hirosawa M, Fujita Y, Parr CJC, Hayashi K, Kashida S, Hotta A, Woltjen K, Saito H.: Cell-type-specific genome editing with a microRNA-responsive CRISPR-Cas9 switch., Nucleic Acids Res. 2017 Jul 27;45(13):e118. doi: 10.1093/nar/gkx309.

Kawasaki S, Fujita Y, Nagaike T, Tomita K, Saito H.: Synthetic mRNA devices that detect endogenous proteins and distinguish mammalian cells., Nucleic Acids Res. 2017 Jul 7;45(12):e117. doi: 10.1093/nar/gkx298.

Kuang Y, Miki K, Parr CJC, Hayashi K, Takei I, Li J, Iwasaki M, Nakagawa M, Yoshida Y, Saito H.: Efficient, Selective Removal of Human Pluripotent Stem Cells via Ecto-Alkaline Phosphatase-Mediated Aggregation of Synthetic Peptides., Cell Chem Biol. 2017 Jun 22;24(6):685-694.e4. doi: 10.1016/j.chembiol.2017.04.010. Epub 2017 May 18.

Parr Callum, Shinya Yamanaka, Hirohide Saito.*: An update on stem cell biology and engineering for brain development., Mol Psychiatry. 2017 Jun;22(6):808-819. doi: 10.1038/mp.2017.66. Epub 2017 Apr 4.

Shibata T, Fujita Y, Ohno H, Suzuki Y, Hayashi K, Komatsu KR, Kawasaki S, Hidaka K, Yonehara S, Sugiyama H, Endo M, Saito H.: Protein-driven RNA nanostructured devices that function in vitro and control mammalian cell fate., Nat Commun. 2017 Sep 14;8(1):540. doi: 10.1038/s41467-017-00459-x.

Tang L, Morris J, Wan J, Moore C, Fujita Y, Gillaspie S, Aube E, Nanda J, Marques M, Jangal M, Anderson A, Cox C, Hiraishi H, Dong L, Saito H, Singh CR, Witcher M, Topisirovic I, Qian SB, Asano K.: Competition between translation initiation factor eIF5 and its mimic protein 5MP determines non-AUG initiation rate genome-wide., Nucleic Acids Res. 2017 Nov 16;45(20):11941-11953. doi: 10.1093/nar/gkx808.

2016

Ohno H, Saito H.: RNA and RNP as Building Blocks for Nanotechnology and Synthetic Biology., Prog Mol Biol Transl Sci. 2016:139:165-85. doi: 10.1016/bs.pmbts.2015.12.004.

Endo K, Hayashi K, Saito H.: High-resolution Identification and Separation of Living Cell Types by Multiple microRNA-responsive Synthetic mRNAs., Sci Rep. 2016 Feb 23:6:21991. doi: 10.1038/srep21991.

Parr CJ, Katayama S, Miki K, Kuang Y, Yoshida Y, Morizane A, Takahashi J, Yamanaka S, Saito H.: MicroRNA-302 switch to identify and eliminate undifferentiated human pluripotent stem cells., Sci Rep. 2016 Sep 9:6:32532. doi: 10.1038/srep32532.

Karagiannis P, Fujita Y, Saito H.: RNA-based gene circuits for cell regulation., Proc Jpn Acad Ser B Phys Biol Sci. 2016;92(9):412-422. doi: 10.2183/pjab.92.412.

2015

Ohno H, Osada E, Saito H.: Design, assembly, and evaluation of RNA-protein nanostructures., Methods Mol Bio, 2015:1297:197-211. doi: 10.1007/978-1-4939-2562-9_14.

Shibata T, Suzuki Y, Sugiyama H, Endo M, Saito H.: Folding RNA-Protein Complex into Designed Nanostructures., Methods Mol Bio. 2015:1316:169-79. doi: 10.1007/978-1-4939-2730-2_14.

Miki K, Endo K, Takahashi S, Funakoshi S, Takei I, Katayama S, Toyoda T, Kotaka M, Takaki T, Umeda M, Okubo C, Nishikawa M, Oishi A, Narita M, Miyashita I, Asano K, Hayashi K, Osafune K, Yamanaka S, Saito H, Yoshida Y.: Efficient Detection and Purification of Cell Populations Using Synthetic MicroRNA Switches., Cell Stem Cell. 2015 Jun 4;16(6):699-711. doi:10.1016/j.stem.2015.04.005. Epub 2015 May 21.

Wroblewska L, Kitada T, Endo K, Siciliano V, Stillo B, Saito H, Weiss R.: Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery., Nat Biotechnol. 2015 Aug;33(8):839-41. doi: 10.1038/nbt.3301. Epub 2015 Aug 3.

Morita M, Onoe H, Yanagisawa M, Ito H, Ichikawa M, Fujiwara K, Saito H, Takinoue M.: Droplet-Shooting and Size-Filtration (DSSF) Method for Synthesis of Cell-Sized Liposomes with Controlled Lipid Compositions., Chembiochem. 2015 Sep 21;16(14):2029-35. doi: 10.1002/cbic.201500354. Epub 2015 Aug 17.

2014

Endo K, Saito H*: Engineering protein-responsive mRNA switch in mammalian cells , Methods Mol Biol., 2014;1111:183-96. doi: 10.1007/978-1-62703-755-6_13. PMID: 24549620

Kashida S, Saito H*: :A three-dimensional design strategy for a protein-responsive shRNA switch,, Methods Mol Biol., 2014;1111:269-86. doi: 10.1007/978-1-62703-755-6_20. PMID: 24549627

Hagiya M*, Konagaya A, Kobayashi S, Saito H, Murata S: Molecular robots with sensors and intelligence, Acc Chem Res., 2014 Jun 17;47(6):1681-90. doi:10.1021/ar400318d. Epub 2014 Jun 6. PMID: 24905779 

Osada E, Suzuki Y, Kumi Hidaka, Hirohisa Ohno, Hiroshi Sugiyama, Masayuki Endo, Saito H*: Engineering RNA-Protein Complexes with Different Shapes for Imaging and Therapeutic Applications , ACS Nano., 2014 Aug 5. [Epub ahead of print] PMID: 25058166

Endo K, Parr C, Saito H: Expanding the synthetic ribonucleoprotein world in cells., Nature Methods, 2014 Oct 30; 11, pages1105–1106

2013

Ohno H, Osada E, Inoue T, and Saito H*: Synthetic RNA-Protein Nanostructures and Their Potential Applications, Review: RNA Nanotechnology and Therapeutics, (ed. Guo, P., CRC press),2013 in press.

Hara T, Saito H*, and Inoue T*: Directed evolution of a synthetic RNA-protein module to create a new translational switch, Chemical Communications 2013 May 10;49(37):3833-5. doi: 10.1039/c3cc38688k.PMID: 23381780 (Selected as the front cover image)

Endo K, Stapleton JA, Hayashi K, Saito H*, and Inoue T*: Quantitative and stimulataneous translational control of distinct mammalian mRNAs, Nucleic Acid Res., 2013 Jul;41(13):e135. doi: 10.1093/nar/gkt347. 

Endo K, Hayashi K, Inoue T*, and Saito H*: A versatile cis-acting inverter module for synthetic translational switches, Nature Communications, 2013, 4:2393, DOI: 10.1038/ncomms3393.

2012

James A. Stapleton, Kei Endo, Yoshihiko Fujita, Karin Hayashi, Masahiro Takinoue, Saito H*, and Tan Inoue*: Feedback Control of Protein Expression in Mammalian Cells by Tunable Synthetic Translational Inhibition, ACS Synthetic Biology, 2012 Mar 16;1(3):83-8. doi: 10.1021/sb200005w. (Selected as a cover article)

Kashida S, Inoue T*, and Saito H*: Three-dimensionally designed protein-responsive RNA devices for cell signaling regulation, Nucleic Acids Research, 2012 Oct;40(18):9369-78. doi: 10.1093/nar/gks668.  (Selected as Featured Articles represent the top 5% of papers)

Ohno H, Osada E, Inoue T, and Saito H*: Synthetic RNA-Protein Nanostructures and Their Potential Applications, Review: RNA Nanotechnology and Therapeutics, (ed. Guo, P., CRC press),2013 in press.

2011

Saito H*, Yoshihiko Fujita, Shunnichi Kashida, Karin Hayashi, and *Tan Inoue: Synthetic human cell fate regulation by protein-driven RNA swiches, Nature Communications, 2011 Jan 18:2:160. doi: 10.1038/ncomms1157.

Hirohisa Ohno, Tetsuhiro Kobayashi, Rinko Kabata, Kei Endo, Takuma Iwasa, Shige Yoshimura, Kunio Takeyasu, Tan Inoue*, and Saito H*: Synthetic RNA-protein complex shaped like an equilateral triangle, Nature Nanotechnology, 2011 Feb;6(2):116-20. doi: 10.1038/nnano.2010.268.

Rei Ohmori, Saito H, Yoshiya Ikawa, Yoshihiko Fujita, Tan Inoue: Self-Replication Reactions Dependent on Tertiary Interaction Motifs in an RNA Ligase Ribozyme, J Mol Evol., 2011 Oct;73(3-4):221-9. doi: 10.1007/s00239-011-9471-2.

Sergii Rudiuk, Saito H, Tomoaki Hara, Tan Inoue, Kenichi Yoshikawa, Damien Baigl:Light-Regulated mRNA Condensation by a Photosensitive Surfactant Works as a Series Photoswitch of Translation Activity in the Presence of Small RNAs, Biomacromolecules,  2011 Nov 14;12(11):3945-51. doi: 10.1021/bm200962s.

2010

Saito H*, Tetsuhiro Kobayashi, Tomoaki Hara, Yoshihiko Fujita, Karin Hayashi, Rie Furushima, and Tan Inoue*: Synthetic Translational Regulation by an L7Ae-Kink-turn RNP Switch, Nature Chemical Biology, 2010 Jan;6(1):71-8. doi: 10.1038/nchembio.273.

2009

Tomoaki Shiohara, Saito H*, and Tan Inoue*: A designed RNA selection: establishment of a stable complex between a target and selectant RNA via two coordinated interactions, Nucleic Acids Res., 2009 Feb;37(3):e23. doi: 10.1093/nar/gkn1012.

Saito H* and Tan Inoue*: Synthetic Biology with RNA motifs, Int. J. Biochem. Cell Biol.,2009 Feb;41(2):398-404. doi: 10.1016/j.biocel.2008.08.017.

Saito H*, Yusho Kato, MAel Le Berre, Ayako Yamada, Tan Inoue, Kenichi Yoshikawa, and Damien Baigl: Time-resolved tracking of a minimum gene expression system reconstituted in giant liposomes, ChemBioChem. 2009 Jul 6;10(10):1640-3. doi: 10.1002/cbic.200900205.

André Estévez－Torres, Cécile Crozatier, Antoine Diguet, Tomoaki Hara, Saito H, Kenichi Yoshikawa, Damien Baigl: Sequence-independent and reversible photocontrol of transcription/expression systems using a photosensitive nucleic acid binder, PNAS, 106 (30): 2009 Jul 28;106(30):12219-23. doi: 10.1073/pnas.0904382106.

Shigeyoshi Matsumura, Rei Ohmori, Saito H, Yoshiya Ikawa, Tan Inoue: Coordinated control of a designed trans-acting ligase ribozyme by a loop-receptor interaction, FEBS Letters, 2009 Sep 3;583(17):2819-26. doi: 10.1016/j.febslet.2009.07.036.

2008

Saito H, Tamiko Minamisawa, Takao Yamori, Kiyotaka Shiba: Motif-programmed artificial protein induces apoptosis in several cancer cells by disrupting mitochondria, Cancer Science, 2008 Feb;99(2):398-406. doi: 10.1111/j.1349-7006.2007.00697.x.

2007

Saito H*, Tamiko Minamisawa, Kiyotaka Shiba*: Motif programming: a microgene-based method for creating synthetic proteins containing multiple functional motifs, Nucleic Acid. Res., 2007;35(6):e38. doi: 10.1093/nar/gkm017.

Saito H*, Tan Inoue: RNA and RNP as new molecular parts in synthetic biology, J. Biotechnol., 2007 Oct 15;132(1):1-7. doi: 10.1016/j.jbiotec.2007.07.952.

Saito H*, Shunichi Kashida, Tan Inoue, Kiyotaka Shiba: The role of peptide motifs in the evolution of a protein network, Nucleic Acid. Res., 2007 Oct; 35(19): 6357–6366. doi: 10.1093/nar/gkm692.

2004

Krishna Ramaswamy, Saito H, Hiroshi Murakami, Kiyotaka Shiba, Hiroaki Suga: Designer Ribozymes: Programming the tRNA Specificity into Flexizyme, J. Am. Chem. Soc., 2004, 126 (37) 11454 –11455.

Saito H, Takako Honma,, Tamiko Minamisawa, Kanami Yamazaki, Tetsuo Noda, Takao Yamori, Kiyotaka Shiba: Synthesis of functional proteins by mixing peptide motifs, Chemistry & Biology, 2004 Jun;11(6):765-73. doi: 10.1016/j.chembiol.2004.03.032.

2003

Hiroshi Murakami, Saito H, Hiroaki Suga: A versatile tRNA aminoacylation catalyst based on RNA, Chemistry & Biology, 2003 Jul;10(7):655-62. doi: 10.1016/s1074-5521(03)00145-5.

2002

Saito H, Hiroaki Suga: Outersphere and innersphere coordinated metal ions in an aminoacyl-tRNA synthetase ribozyme, Nucleic Acid. Res., 2002 Dec 1;30(23):5151-9. doi: 10.1093/nar/gkf641.

2001

Saito H, Dimitrios Kourouklis, Hiroaki Suga: An in vitro evolved precursor tRNA with aminoacylation activity, The EMBO Journal, 2001 Apr 2; 20(7): 1797–1806.

Saito H, Hiroaki Suga: A ribozyme exclusively aminoacylates the 3´- hydroxyl group of the tRNA terminal adenosine, J. Am. Chem. Soc., 2001, 123, 29, 7178–7179.

Saito H, Kimitsuna Watanabe, Hiroaki Suga: Concurrent molecular recognition of both the amino acid and tRNA by a ribozyme, RNA, 2001 Dec; 7(12): 1867–1878.