IQB Handbook

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15ACHIEVEMENTPUBLICATIONLim B, Heist T, Levine M, Fukaya T. Visualization of Transvection in Living Drosophila Embryos. Mol Cell. 2018 Apr 19;70(2):287-296.e6. DOI:10.1016/j.molcel.2018.02.029Eritano AS, Bromley CL, Bolea Albero A, Schütz L, Wen FL, Takeda M, Fukaya T, Sami MM, Shibata T, Lemke S, Wang YC. Tissue-Scale Mechanical Coupling Reduces Morphogenetic Noise to Ensure Precision during Epithelial Folding. Dev Cell. 2020 Apr 20;53(2):212-228.e12. DOI: 10.1016/j.devcel.2020.02.012Yokoshi M, Segawa K, Fukaya T. Visualizing the Role of Boundary Elements in Enhancer-Promoter Communication. Mol Cell. 2020 Apr 16;78(2):224-235.e5. DOI: 10.1016/j.molcel.2020.02.007(1) Expression of pair-rule genes eve (red) and ftz (green) in the early Drosophila embryo (2) Live imaging of transcription activities in living Drosophila embryosFUKAYATAKASHIPH.D. (2014) UNIVERSITY OF TOKYOPOSTDOCTORAL FELLOW(2014) UNIVERSITY OF CALIFORNIA BERKELEYJSPS POSTDOCTORAL FELLOW (2015) UNIVERSITY OF CALIFORNIA BERKELEYPOSTDOCTORAL FELLOW (HFSP FELLOW) (2015) PRINCETON UNIVERSITYLECTURER (2017)INSTITUTE OF MOLECULAR AND CELLULAR BIOSCIENCES, THE UNIVERSITY OF TOKYOLECTURER (2018)IQB / INSTITUTE FOR QUANTITATIVE BIOSCIENCES, THE UNIVERSITY OF TOKYO●MEMBER■ LECTURER :FUKAYA TAKASHI■ RESEARCH ASSOCIATE :YOKOSHI MOE■ PROJECT RESEARCHER : KAWASAKI KOJI■ PROJECT ACADEMIC SUPPORT STAFF : TAKISHITA HITOMI PUBLICATIONPUBLICATIONnhancers are regulatory DNAs that instruct spatiotemporal patterning of gene expression in response to developmental timing and environ-mental signals. By integrating information from sequence specific transcription factors and co-activators, enhancers control when and where genes should be transcribed to establish localized patterns of gene expres-sion during animal development. Recent whole genome studies estimated that the human genome contains approximately 400,000 enhancers, suggesting that a typical human gene is regulated by ～20 enhancers (ENCODE Consortium, Nature 2011). Muta-tion in enhancers often causes dysregulation in transcriptional program, which can ultimately lead to human diseases such as cancer. It has also been reported that diversi-fication of enhancer function is a major source ofphenotypic polymorphism in population. While biological importance of transcriptional control by enhancers is becoming increasingly clear in recent years, very little is known about the fundamental mechanism of enhancer function. Especially, the nature of enhancer-promoter communication and its dynamics remain as an outstanding mystery in the central dogma since the first discover of prototypic SV40 enhancer more than 35 years ago (Banerji et al., Cell 1981). Our lab uses quantitative live imaging method to visualize dynamics of enhancer-promoter communication and resulting gene expression in developing Drosophila embryos. By combining cutting-edge new technologies such as genome editing and optogenetics, we aim to obtain comprehensive understanding of how enhancers control transcriptional activities in time and space during development.E