IQB Handbook

13/100

11ACHIEVEMENTPUBLICATIONDeardorff MA, Bando M, Nakato R, Watrin E, Itoh T, Minamino M, Saitoh K, Komata M, Katou Y, Clark D, Cole KE, De Baere E, Decroos C, Di Donato N, Ernst S, Francey LJ, Gyftodimou Y, Hirashima K, Hullings M, Ishikawa Y, Jaulin C, Kaur M, Kiyono T, Lombardi PM, Magnaghi-Jaulin L, Mortier GR, Nozaki N, Petersen MB, Seimiya H, Siu VM, Suzuki Y, Takagaki K, Wilde JJ, Willems PJ, Prigent C, Gillessen-Kaesbach G, Christianson DW, Kaiser FJ, Jackson LG, Hirota T, Krantz ID, Shirahige K. HDAC8 mutations in Cornelia de Lange syndrome affect the cohesin acetylation cycle. Nature. 2012 Sep 13;489(7415):313-7. DOI:10.1038/nature11316Izumi K, Nakato R, Zhang Z, Edmondson AC, Noon S, Dulik MC, Rajagopalan R, Venditti CP, Gripp K, Samanich J, ZackaiEH, Deardorff MA, Clark D, Allen JL, Dorsett D, Misulovin Z, Komata M, Sando M, Kaur M, Katou Y, Shirahige K*, Krantz ID*. (Shared Corresponding Authors)Germline gain-of-function mutations in AFF4 cause a developmental syndrome functionally linking the super elongation complex and cohesin. Nat Genet. 2015 Apr;47(4):338-44. DOl:10.1038/ng.3229Wendt KS, Yoshida K, Itoh T, Bando M, Koch B, Schirghuber E, Tsutsumi S, Nagae G, Ishihara K, Mishiro T, Yahata K, Imamoto F, Aburatani H, Nakao M, Imamoto N, Maeshima K, Shirahige K*, Peters JM*.(Shared Corresponding Authors) Cohesin mediates transcriptional insulation by CCCTC-binding factor. Nature. 2008 Feb 14;451(7180):796-801. DOI: 10.1038/nature06634A model of cohesin complex by netsuke. The two dragons represent proteins SMC1 and 3, the carp represents protein STAG, and the waterfall (with the carp) represents RAD 21. The interaction between RAD 21 and SMC3 is regulated by acetylation as shown by the lock. NIPBL, a mysterious protein whose function is largely unknown, exists in the inside of the ring. Because of the ATPase activity of NIPBL, cohesin is known to function as a DNA motor, and DNA is incorporated into this ring. On the reverse side of the netsuke (the far right figure), Raijin is depicted, and the drum possessed by Raijin represents chromosome leading to the front of netsuke (the far left figure).SHIRAHIGEKATSUHIKOPH.D. (1993) OSAKA UNIVERSITYPROFESSOR (2007) TOKYO INSTITUTE OF TECHNOLOGYPROFESSOR (2010) INSTITUTE OF MOLECULAR AND CELLULAR BIOSCIENCES, THE UNIVERSITY OF TOKYODIRECTOR (2017-2018) INSTITUTE OF MOLECULAR AND CELLULAR BIOSCIENCES, THE UNIVERSITY OF TOKYODIRECTOR (2018-) IQB / INSTITUTE FOR QUANTITATIVE BIOSCIENCES, THE UNIVERSITY OF TOKYO●MEMBER■ PROFESSOR :SHIRAHIGE KATSUHIKO■ ASSOCIATE PROFESSOR :SUTANI TAKASHI■ LECTURER :BANDO MASASHIGE■ RESEARCH ASSOCIATE : FUJIKI KATSUNORI■ PROJECT RESEARCH ASSOCIATE, URA :NAKAGAWA YURI■ PROJECT RESEARCH ASSOCIATE : SAKATA TOYONORI■ PROJECT RESEARCHER :YOSHIMURA ATSUNORI■ TECHNICAL SPECIALIST :NAKAGAWA KEIKO■ ASSISTANT CLERK :KIKUCHI KIRARAPUBLICATIONPUBLICATIONhe main theme of my laboratory is to study how chromosome, the blueprint of life, is structured, how information is input to and output from chromosome, and how information on chromosome is transmitted to next generation. Therefore, we are conducting research utilizing genomic, biochemical, and genetic technologies.I graduated the College of Liberal Arts in the University of Tokyo. I was a graduate of a laboratory of molecular spectroscopy, which is not related to living things, but I entered a laboratory of genetics from graduate school, and I have been studying eukaryotic DNA replica-tion. It was the dawn of genome sequencing project of model organisms when I was in graduate school and I contributed to the sequencing project of the budding yeast Saccha-romyces cerevisiae (which is also called baker's yeast or brewer's yeast), and I have been involved in the research of genomic DNA replication of the budding yeast. I learned the importance of genomic analysis, the importance of massive and parallel study at that time, and this has become a basis of my research since then. I am enchanted by utilizing and developing so-called genome wide technologies based on DNA chips and next-generation sequencers, which offer us comprehensive analysis technolo-gies for understanding various aspects of chromosome functions such as replication and transcription at whole genome level.Currently, my laboratory is focusing on research of specific rare diseases in humans to elucidate the mechanisms that regulate the transcription and replication. In particular, I am pretty much interested in protein complexes called cohesin and condensin that control the local and global structure of the genome and play their roles in various chromosome functions. How do these proteins act locally and define the overall structure of the chromosome? How can muta-tions in these proteins in human rare diseases affect their activities? Transcription and replication may seem like very basic field of life science and everyone may feel like there are not so much things remained to be studied, but there are still many topics that are surprisingly unclear. I am eager to solve the enigmas of chromosome structure and function through cutting-edge genome technologies.T