Publications

 1. M. Takahashi, Y. Kondou and C. Toyoshima: Interdomain communication in calcium pump as revealed in the crystal structures with transmembrane inhibitors. Proc. Nat. Acad. Sci. USA. 104 (2007) 5800-5805. [pubmed]

 2. G. Inesi, D. Lewis, H. Ma, A. Prasad and C. Toyoshima: Concerted conformational effects of Ca²⁺ and ATP are required for activation of sequential reactions in the Ca²⁺ ATPase (SERCA) catalytic cycle. Biochemistry 46 (2006) 13769-13778. [pubmed]

 3. Y. Sugita, N. Miyashita, T. Yoda, M. Ikeguchi and C. Toyoshima: Structural changes of the cytoplasmic domain of phospholamban by phosphorylation at Ser16: A molecular dynamics study. Biochemistry 45 (2006) 11752-11761. [pubmed]

 4. M. Picard, C. Toyoshima and P. Champeil: Effects of inhibitors on luminal opening of Ca²⁺ binding sites in an E2P-like complex of sarcoplasmic reticulum Ca²⁺-ATPase with Be²⁺-fluoride. J. Biol. Chem. 281 (2006) 3360-3369. [pubmed]

 5. K. Obara, N. Miyashita, C. Xu, I. Toyoshima, Y. Sugita, G. Inesi and C. Toyoshima: Structural role of countertransport revealed in Ca²⁺ pump crystal structure in the absence of Ca²⁺. Proc. Nat. Acad. Sci. USA. 102 (2005) 14489-14496. [pubmed]

 6. H. Ma, D. Lewis, C. Xu, G. Inesi and C. Toyoshima: Functional and structural roles of critical amino acids within the"N", "P", and "A" domains of the Ca2+ ATPase (SERCA) headpiece. Biochemistry. 44 (2005) 8090-8100. [pubmed]

 7. M. Picard, C. Toyoshima and P. Champeil: The average conformation at micromolar [Ca²⁺] of Ca²⁺-ATPase with bound nucleotide differs from that adopted with the transition state analog ADP.AlFx or with AMPPCP under crystallization conditions at millimolar [Ca²⁺]. J. Biol. Chem. 280 (2005) 18745-18754. [pubmed]

 8. Y. Sugita, N. Miyashita, M. Ikeguchi, A. Kidera, and C. Toyoshima: Protonation of the acidic residues in the transmembrane cation-binding sites of the Ca²⁺ pump. J. Am. Chem. Soc. 127 (2005) 6150-6151. [pubmed]

 9. C. Toyoshima, H. Nomura and T. Tsuda: Lumenal gating mechanism revealed in calcium pump crystal structures with phosphate analogues. Nature 432 (2004) 361-368. [pubmed] [reprint] [Supplement]

10. C. Toyoshima and T. Mizutani: Crystal structure of the calcium pump with a bound ATP analogue. Nature 430 (2004) 529-535. [pubmed] [reprint] [Supplement]

11. C. Xu, H. Ma, G. Inesi, M.K. Al-Shawi and C. Toyoshima: Specific structural requirement for the inhibitory effect of thapsigargin on the Ca²⁺ ATPase SERCA. J. Biol. Chem. 279 (2004) 17973-17979. [pubmed]

12. M. Asahi, Y. Sugita, K. Kurzydlowski, S. D. Leon, M. Tada, C. Toyoshima, and D.H. MacLennan: Sarcolipin regulates sarco(endo)plasmic reticulum Ca²⁺-ATPase(SERCA) by binding to transmembrane helices alone or in association with phospholamban. Proc. Nat. Acad. Sci. USA 100 (2003) 5040-5045. [pubmed]

13. K. Yonekura, C. Toyoshima, S. Maki-Yonekura, and K. Namba: GUI programs for processing individual images in early stages of helical image reconstruction-for high-resolution structure analysis. J. Struct. Biol. 144 (2003) 184-194. [pubmed]

14. C. Toyoshima, H. Nomura and Y. Sugita: Structural basis of ion pumping by Ca²⁺-ATPase of sarcoplasmic reticulum. FEBS Letters 555 (2003) 106-110. [pubmed]

15. H. Ma, G. Inesi and C. Toyoshima: Substrate-induced conformational fit and headpiece closure in the Ca²⁺ATPase (SERCA). J. Biol. Chem. 278 (2003) 28938-28943. [pubmed]

16. M. Asahi, Y. Sugita, K. Kurzydlowski, S. De Leon, M. Tada, C. Toyoshima and D.H. MacLennan: Sarcolipin regulates sarco(endo)plasmic reticulum Ca²⁺-ATPase (SERCA) by binding to transmembrane helices alone or in association with phospholamban. Proc. Nat. Acad. Sci. USA. 100 (2003) 5040-5045. [pubmed]

17. C. Toyoshima, M. Asahi, Y. Sugita, R. Khanna, T. Tsuda and D.H. MacLennan: Modeling of the inhibitory interaction of phospholamban with the Ca²⁺ATPase. Proc. Nat. Acad. Sci. USA 100 (2003) 467-472. [pubmed]

18. C. Toyoshima and H. Nomura: Structural changes in the calcium pump accompanying the dissociation of calcium. Nature 418 (2002) 605-611. [pubmed] [reprint] [Supplement]

19. H. Ogawa and C. Toyoshima: Homology modeling of the cation binding sites of Na⁺K⁺-ATPase. Proc. Nat. Acad. Sci. USA. 99 (2002) 15977-15982. [pubmed]

20. S. Hua, H. Ma, D. Lewis, G. Inesi and C. Toyoshima: Functional role of “N” (Nucleotide) and “P” (Phosphorylation) domain interactions in the sarcoplasmic reticulum (SERCA) ATPase. Biochemistry 41(2002) 2264-2272. [pubmed]

21. S. Hua, G. Inesi, H. Nomura and C. Toyoshima: Fe²⁺-catalyzed oxidation and cleavage of sarcoplasmic reticulum ATPase reveals Mg²⁺ and Mg²⁺-ATP sites. Biochemistry 41 (2002) 11405-11410. [pubmed]

22. J. V. Møller, G. Lenoir, C. Marchand, C. Montigny, M. le Maire, C. Toyoshima, B. S. Juul and P. Champeil: Calcium transport by sarcoplasmic reticulum Ca²⁺-ATPase: role of the A-domain and its C-terminal link with the transmembrane region. J. Biol. Chem. 277 (2002) 38647-38659. [pubmed]

23. S. Danko, K. Yamasaki, T. Daiho, H. Suzuki, C. Toyoshima: Organization of cytoplasmic domains of sarcoplasmic reticulum Ca²⁺-ATPase in E1P and E1ATP states: a limited proteolysis study. FEBS letters 505 (2001) 129-135. [pubmed]

24. S. Danko, T. Daiho, K. Yamasaki, M. Kamidochi, H. Suzuki, C. Toyoshima: ADP-insensitive phosphoenzyme intermediate of sarcoplasmic reticulum Ca²⁺-ATPase has a compact conformation resistant to proteinase K, V8 protease and trypsin. FEBS letters 489 (2001) 277-282. [pubmed]

25. Z. Zhang, D. Lewis, C. Sumbilla, G. Inesi, and C. Toyoshima: The role of the M6-M7 Loop (L67) in stabilization of the phosphorylation and Ca²⁺ binding domains of the sarcoplasmic reticulum Ca²⁺-ATPase (SERCA). J. Biol. Chem. 276 (2001) 15232-15239. [pubmed]

26. C. Toyoshima, M. Nakasako, H. Nomura and H. Ogawa: Crystal structure of the calcium pump of sarcoplasmic reticulum at 2.6 Å resolution. Nature 405 (2000) 647-655. [pubmed] [reprint]

27. C. Toyoshima: Structure determination of tubular crystals of membrane proteins. I. Indexing of diffraction patterns. Ultramicroscopy 84 (2000) 1-14. [pubmed]

28. K. Yonekura and C. Toyoshima: Structure determination of tubular crystals of membrane proteins. II. Averaging of tubular crystals of different helical classes. Ultramicroscopy 84 (2000) 15-28. [pubmed]

29. K. Yonekura and C. Toyoshima: Structure determination of tubular crystals of membrane proteins. III. Solvent flattening. Ultramicroscopy 84 (2000) 29-45. [pubmed]

30. H. Ogawa, T. Haga and C. Toyoshima: Soluble P-type ATPase from an archaeon, Methanococcus jannaschii. FEBS letters 471 (2000) 99-102. [pubmed]

31. S. Hua, G. Inesi and C. Toyoshima: Distinct topologies of mono-and decavanadate binding and photo-oxidative cleavage in the sarcoplasmic reticulum ATPase. J. Biol. Chem. 275 (2000) 30546-30550. [pubmed]

32. Z. Zhang, D. Lewis, C. Strock, G. Inesi, M. Nakasako, H. Nomura and C. Toyoshima: Detailed characterization of the cooperative mechanism of Ca²⁺ binding and catalytic activation in the Ca²⁺ transport (SERCA) ATPase. Biochemistry 39 (2000) 8758-8767. [pubmed]

33. K. Hirose, U. Henningsen, M. Schliwa, C. Toyoshima, T. Shimizu and M. Alonso, R.A.Cross and L.A. Amos: Structural comparison of dimeric Eg5, Neurospora Kinesin (Nkin) and Ncd Head-Nkin Neck chimera with conventional Kinesin. EMBO Journal, 19 (2000) 5308-5314. [pubmed]

34. K. Mayanagi, T. Ishikawa, C. Toyoshima, Y. Inoue and K. Nakazato: Three-dimensional electron microscopy of the photosystem II core complex. J. Struct. Biol. 123 (1998) 211-224.

35. H. Ogawa, D.L. Stokes, H. Sasabe and C. Toyoshima: Structure of the Ca²⁺ pump of sarcoplasmic reticulum: A view along the lipid bilayer at 9-Å resolution. Biophys. J. 75 (1998) 41-52. [pubmed]

36. P. Zhang, C. Toyoshima, K. Yonekura, N.M. Green and D.L. Stokes: Structure of the calcium pump from sarcoplasmic reticulum at 8 Å resolution. Nature 392 (1998) 835-839. [pubmed] [reprint]

37. K. Yonekura, D.L. Stokes, H. Sasabe and C. Toyoshima: The ATP binding site of Ca²⁺-ATPase revealed by electron image analysis. Biophys. J. 72 (1997) 997-1005. [pubmed]

38. K. Tani, H. Sasabe and C. Toyoshima: A set of computer programs for determining defocus and astigmatism in electron images. Ultramicroscopy 65 (1996) 31-44.

39. T. Akiba, C. Toyoshima, T. Matsunaga, M. Kawamoto, T. Kubota, K. Fukuyama, K. Namba and H. Matsubara: Three-dimensional structure of bovine cytochrome bc1 complex by electron cryomicroscopy and helical image reconstruction. Nature Struct. Biol. 3 (1996) 553-561. [pubmed]

40. G-W. Choeng, H.S. Young, H. Ogawa, C. Toyoshima D.L. Stokes: Lamellar stacking in three-dimensional crystals of Ca²⁺-ATPase from sarcoplasmic reticulum. Biophys. J. 70 (1996) 1689-1699. [pubmed]

41. K. Nakazato, C. Toyoshima, I. Enami and Y. Inoue: Two-dimensional Crystallization and cryo-electron microscopy of photosystem II. J. Mol. Biol. 257 (1996) 225-232. [pubmed]

42. M. Nakasako, T. Ueki, C. Toyoshima and Y. Umeda: A crystal mounting device made from a capillary tube for cryogenic macromolecular crystallography. J. Appl. Cryst. 28 (1995) 856-857.

43. Y. Mimori, I. Yamashita, K. Murata, Y. Fujiyoshi, K. Yonekura, C. Toyoshima and K. Namba: The Structure of the R-type straight flagellar filament of Salmonella at 9 Å resolution by electron cryomicroscopy. J. Mol. Biol. 249 (1995) 69-87. [pubmed]

44. C. Toyoshima, H. Sasabe and D.L. Stokes: Three-dimensional cryo-electron microscopy of the calcium ion pump in the sarcoplasmic reticulum membrane. Nature 362 (1993) 469-471. [pubmed]

45. C. Toyoshima, K. Yonekura and H. Sasabe: Contrast transfer for the frozen hydrated specimen. II: Amplitude contrast at very low frequencies. Ultramicroscopy 48 (1993) 165-176.

46. A. Sato, T. Furuno, C. Toyoshima and H. Sasabe: Two-dimensional crystallization of catalase on a monolayer film of poly (1-benzyl-L-histidine) spread at the air/water interface. Biochim. Biophys. Acta 1162 (1993) 54-60. [pubmed]

47. K. Yamane, C. Toyoshima and S. Nishimura: Ligand-induced functions of the epidermal growth factor receptor require the positively charged region asymmetrically distributed across plasma membrane. Biochem. Biophys. Res. Comm. 184 (1992) 1301-1310. [pubmed]

48. C. Toyoshima and N. Unwin: Three-dimensional structure of the acetylcholine receptor by cryo-electron microscopy and helical reconstruction. J. Cell. Biol. 111 (1990) 2623-2635. [pubmed]

49. C. Toyoshima: On the use of holey grids in electron crystallography. Ultramicroscopy 30 (1989) 439-444.

50. Y.Y. Toyoshima, C. Toyoshima and J.A. Spudich: Bidirectional movement of actin filaments along tracks of myosin heads. Nature 341 (1989) 154-156. [pubmed]

51. T. Miyanishi, C. Toyoshima, T. Wakabayashi and G. Matsuda: Electron microscopic study on the location of 23K and 50K fragments in skeletal myosin head. J. Biochem. 103 (1988) 458-462. [pubmed]

52. C. Toyoshima and N. Unwin: Contrast transfer for the frozen hydrated specimen: determination from pairs of defocused images. Ultramicroscopy 25 (1988) 279-292. [pubmed]

53. N. Unwin, C. Toyoshima and E. Kubalek: Arrangement of the acetylcholine receptor subunits in the resting and desensitized states, determined by cryo-electron microscopy of crystallized Torpedo postsynaptic membranes. J. Cell Biol.107 (1988) 1123-1138. [pubmed]

54. C. Toyoshima and N. Unwin: Ion channel of the acetylcholine receptor reconstructed from images of postsynaptic membranes. Nature 336 (1988) 247-250. [pubmed]

55. Y.Y. Toyoshima, S.J. Kron, E.M. McNally, K.R. Niebling, C. Toyoshima and J.A. Spudich: Myosin Subfrgment-1 is sufficient to move actin filaments in vitro. Nature 328 (1987) 536-539. [pubmed]

56. M. Tokunaga, K. Sutoh, C. Toyoshima and T. Wakabayashi: Location of the ATPase site of myosin determined by three-dimensional electron microscopy. Nature 329 (1987) 635-638. [pubmed]

57. C. Toyoshima and T. Wakabayashi: Three-dimensional image analysis of the complex of thin filaments and myosin molecules from skeletal muscle. IV. Reconstitution from minimal- and high-dose images of Actin-tropomyosin-myosin Subfragment-1 Complex. J. Biochem., 97 (1985) 219-243. [pubmed]

58. C. Toyoshima and T. Wakabayashi: Three-dimensional image analysis of the complex of thin filaments and myosin molecules from skeletal muscle. V. assignment of actin in the actin-tropomyosin-myosin Subfragment-1 complex. J. Biochem., 97 (1985) 245-263. [pubmed]

59. Y. Yano, T. Mohri, C. Toyoshima and T. Wakabayashi: Molecular structure of dynein arms. J. Submicroscopic Cytol., 15 (1983) 211-215.

60. T. Wakabayashi and C. Toyoshima: Three-dimensional image analysis of the complex of thin filaments and myosin molecules from skeletal muscle. II. The multi-domain structure of Actin-myosin S1 complex. J. Biochem, 90 (1981) 683-701. [pubmed]

61. C. Toyoshima and T. Wakabayashi: Three-dimensional image analysis of the complex of thin filaments and myosin molecules from skeletal muscle. I. Tilt angle of myosin Subfragment-1 in rigor complex. J. Biochem., 86, (1979) 1887-1890. [pubmed]

Review articles

 1  C. Toyoshima: Ion pumping by calcium ATPase of sarcoplasmic reticulum. In “Regulatory mechanisms of striated muscle contraction. (eds. S. Ebashi & I. Ohtsuki), pp295-303, Springer 2007 [pubmed]

 2  C. Toyoshima and G. Inesi: Structural basis of ion pumping by Ca²⁺-ATPase of the sarcoplasmic reticulum. Ann. Rev. Biochem. 73 (2004) pp. 269-92. [pubmed]

 3  G. Inesi and C. Toyoshima: Catalytic and transport mechanism of the sarco-(endo)plasmic reticulum Ca²⁺-ATPase (SERCA). In” Handbook of ATPases: Biochemistry, Cell Biology, Pathophysiology” eds. M. Futai, Y. Wada and J. H. Kaplan, Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, (2004) pp. 63-87.

 4  C. Toyoshima: Calcium pump (ATPase) of sarcoplasmic reticulum. In “Handbook of Metalloproteions” Vol. 3 eds. Albrecht Messerschmidt, W. Bode and M. Cygler, John Wiley & Sons, Ltd, Chichester, (2004) pp. 667-676.

 5. C. Toyoshima and Y. Sugita: ER/SR Calcium Pump: Structure. In “Encyclopedia of Biological Chemistry Vol. 2”, Elsevier Inc, (2004) pp. 61-65.

 6. C. Toyoshima, H. Nomura and Y. Sugita: Crystal structures of Ca²⁺-ATPase in various physiological states. In “Na, K-ATPase and Related Cation Pumps: Structure, Function, and Regulatory Mechanisms (Annals of The New York Academy of Sciences Vol. 986)” eds. P.L. Jorgensen, S.J.D. Karlish and A.B. Maunsbach, New York (2003) pp.1-8.

 7. G. Inesi, H. Ma, S. Hua and C. Toyoshima: Characterization of Ca²⁺ ATPase residues involved in substrate and cation binding. In “Na, K-ATPase and Related Cation Pumps: Structure, Function, and Regulatory Mechanisms (Annals of The New York Academy of Sciences Vol. 986)” eds. P.L. Jorgensen, S.J.D. Karlish and A.B. Maunsbach, New York (2003) pp.63-71.

 8  G. Inesi, C. Toyoshima: The Ca²⁺ pump of sarco-and endoplasmic reticulum membranes. In “Calcium Homeostasis (Topics in Biological Inorganic Chemistry Vol. 3)” eds. E. Carafoli and J. Krebs, Springer-Verlag, Berlin (2000) pp. 143-154.

 9  C. Toyoshima, M. Nakasako and H. Nomura: Three-dimensional structural study of Ca²⁺-ATPase from sarcoplasmic reticulum. Na/K-ATPase and Related ATPases, eds. K. Taniguchi and S. Kaya, Elsevier, (2000) pp193-196.

10. C. Toyoshima and K. Tani: Electron crystallography of thin protein crystals. In “ Electron Microscopy 1998”, eds. H.A.C. Benavides and M.J.Yacaman, Institute of Physics Publishing, Bristol and Philadelphia (1998) pp.395-396.

11. C. Toyoshima and H. Ogawa: Cryo-electron microscopy of ion channels and pumps. In “Progress in Cell Research Vol. 6” eds. M. Sokabe et al., Elsevier Science BV (1997) pp. 63-73.

12. C. Toyoshima: Three-dimensional cryo-electron microscopy of calcium ion pump. In “MEMBRANE PROTEINS: structure, function and expression control.” eds. N. Hamasaki and K. Mihara, Kyushu University Press, Japan and S. Karger AG, Switzerland. (1997) pp. 247-255.

13. K. Nakazato, C. Toyoshima, I. Enami and Y. Inoue: Formation and characterization of 2D crystals of spinach PSII. In “Photosynthesis;from Light to Biosphere” Vol. III . ed. P.Mathis, Kluwer Academic Publishers, Netherlands. (1995) pp. 317-320.

14. D.L. Stokes, N.M. Green and C. Toyoshima: Structure studies of Ca²⁺-ATPase by Cryoelectron Microscopy. In “The Sodium Pump: Structure, Mechanisms, Hormonal control and its role in disease”. eds. Bamberg and Schoner, W.Springer, New York. (1994) pp. 120-130.

15. T. Wakabayashi, C. Toyoshima and E. Katayama: Image analysis of the complex of Actin-Tropomyosin and myosin Subfragment-1. In “Contractile Mechanisms in Muscle” (1984) pp.21-27, Plenum.

16. T. Wakabayashi, C. Toyoshima and M. Hosoi: Image analysis of actin-tropomyosin-S1. In “ACTIN: Its Structure and Function in Muscle and Non-Muscle Cells” (1983) pp. 27-34, Academic Press Australia.

17. Y. Yano, T. Mohri, C. Toyoshima and T. Wakabayashi: Molecular composition and structure of dynein arms. In “Biological Function of Microtubules and Related Structures” (eds. Sakai et al.) (1982) pp. 125-135, Academic Press.