primer sequence
| ベクター | 会社 | 名前 | FW | 名前 | REV | コメント |
| 培養細胞発現 | pEGFP-C | CLONTECH | GFP
C FW | CATGGTCCTGCTGGAGTTCGTG | GFP
C rev | | |
| pEGFP-N | CLONTECH | CMV
FW | CGCAAATGGGCGGTAGGCGTG | GFP
N rev | CGTCGCCGTCCAGCTCGACCAG | |
| pDsRED2 C1 | CLONTECH | DsRed
FW | AGCTGGACATCACCTCCCACAACG | GFP
C rev | | |
| hrGFP-N1 | STRATAGENE | hrGFP
FW | AGCTGACCAGCCTGGGCAAG | | | |
| | | CMV FW | CGCAAATGGGCGGTAGGCGTG | | | |
| PCDNA3.1 | INVITROGEN | T7(short) | TAATACGACTCACTATAGGG | BGH
rev | TAGAAGGCACAGTCGAGG | |
| pMkit neo | | pMkit
FW | GCTCCTCAGTGGATGTTGCC | pMkit
rev | CGCAATCACAGGTTTACACC | |
| pCS2+ | | CMV
FW | CGCAAATGGGCGGTAGGCGTG | T7? | | |
| | | | | | | |
| | | | | | | |
| adenoX | pshuttle | CLONTECH | pshuttle
FW | CACTGCTTACTGGCTTATCG | pshuttle
rev | AGATGGCTGGCAACTAGAAGG | |
| | | CMV FW | CGCAAATGGGCGGTAGGCGTG | | | |
| | | | | | | |
| RETRO | pMX(ires
puro) | | pMX FW �@ | | pMX
rev �@ | | |
| pMIG(ires
gfp) | 宮島研より | pMX
FW �@ | | | | |
| | | | | | | |
| ルシフェラーゼ | pGL3 | promega | pGL3
FW(RVprimer3) | CTAGCAAAATAGGCTGTCC | luc
rev(GL primer2) | CTTTATGTTTTTGGCGTCTTCC | |
| pGL2 | promega | pGL2
FW(GLprimer1) | TGTATCTTATGGTACTGTAACTG | luc
rev(GL primer2) | CTTTATGTTTTTGGCGTCTTCC | |
| pSP-luc | promega | sp6 | GATTTAGGTGACACTATAG | luc
rev(GL primer2) | CTTTATGTTTTTGGCGTCTTCC | |
| | | | | | | |
| | | | | | | |
| pBLCAT2 | | | | | | |
| twohybrid | pACT2 | CLONTECH | | | | | |
| pGAD10 | CLONTECH | GAD
FW | TACCACTACAATGGATG | GAD
rev | AATTGAGATGGTGCACG | |
| pGAD424 | CLONTECH | GAD
FW | TACCACTACAATGGATG | GAD
rev | AATTGAGATGGTGCACG | |
| pGADT7 | CLONTECH | GAD
FW | TACCACTACAATGGATG | GAD
rev | AATTGAGATGGTGCACG | |
| pBD GAL4 cam | STRATAGENE | primer1 | TCATCGGAAGAGAGTAG | pBD
rev | CATAAATCATAAGAAATTCG | |
| pGBKT7 | CLONTECH | primer1 | TCATCGGAAGAGAGTAG | pBD
rev | CATAAATCATAAGAAATTCG | |
| pGBT9 | CLONTECH | primer1 | TCATCGGAAGAGAGTAG | pBD
rev | CATAAATCATAAGAAATTCG | |
| | | | | | | |
| 大腸菌発現 | pGEX | PHARMACIA | GEX
FW | GGGCTGGCAAGCCACGTTTGGTG] | GEX
rev | CCGGGAGCTGCATGTGTCAGAGG | |
| Pmal-P2 | NEB | malE | | | | |
| pTrcHis | INVITROGEN | pTrcHis
FW | GAGGTATATATTAATGTATCG | | | pTrcHis
A, B, & C. |
| | | | | | | |
| クローニング | pBS2 SK(-) | STRATAGENE | T7(long) | GTAATACGACTCACTATAGGGC | T3 | ATTAACCCTCACTAAAGGGA | |
| | | SK | CGGCCGCTCTAGAACTAGTGGATC | KS | CCTCGAGGTCGACGGTATCG | |
| | | M13-21 | TGTAAAACGACGGCCAGT | M13
rev | CAGGAAACAGCTATGACC | |
| pCMV sport2 | | | | | | |
| pSTBlue-1 | | | | | | |
| pT7Blue | | T7(short) | TAATACGACTCACTATAGGG | | | |
| | | | | | | |
| | | | | | | |
| | | | | | | |
| pGEM | promega | T7(long) | GTAATACGACTCACTATAGGGC | sp6 | GATTTAGGTGACACTATAG | |