Trinitrophenyl derivatives bind differently from parent adenine nucleotides to Ca²⁺-ATPase in the absence of Ca²⁺
PNAS 108 (2011) 1833-1838
C. Toyoshima, S. Yonekura, J. Tsueda, S. Iwasawa

Relationship between Ca²⁺-affinity and shielding of bulk water in the Ca²⁺-pump from molecular dynamics simulations
PNAS 107 (2010) 21465-21469
Y. Sugita, M. Ikeguchi, C. Toyoshima

Crystal structure of the sodium-potassium pump at 2.4 A resolution.
Nature 459 (2009) 446-450
T. Shinoda, H. Ogawa, F. Cornelius, C. Toyoshima

Crystal structure of the sodium-potassium pump (Na⁺,K⁺-ATPase) with bound potassium and ouabain
PNAS 106 (2009) 13742-13747
H. Ogawa, T. Shinoda, F. Cornelius, C. Toyoshima

How processing of aspartylphosphate is coupled to lumenal gating of the ion pathway in the calcium pump
PNAS 104 (2007) 19831-19836
C. Toyoshima, Y. Norimatsu, S. Iwasawa, T. Tsuda, H. Ogawa

Interdomain communication in calcium pump as revealed in the crystal structures with transmembrane inhibitors
PNAS 104 (2007) 5800-5805
M. Takahashi, Y. Kondou, C. Toyoshima

Lumenal gataing mechanism revealed in calcium pump crystal structures with phosphate analogues
Nature 432 (2004) 361-368
C. Toyoshima, H. Nomura & T. Tsuda
SI

Crystal structure of the calcium pump with a bound ATP analogue
Nature 430 (2004) 529-535
C. Toyoshima and T. Mizutani
SI

Review
Annual Reviews of Biochemsitry (2004)

Structural changes in the calcium pump accompanying the dissociation of calcium
Nature 418 (2002)
Chikashi Toyoshima & Hiromi Nomura
SI

Crystal structure of the calcium pump of sarcoplasmic reticulum at 2.6 A resolution
Nature 405 (2000)
Chikashi Toyoshima, Masayoshi Nakasako, Hiromi Nomura & Haruo Ogawa

Structure of the calcium pump from sarcoplasmic reticulum at 8-A resolution
Nature 392 (1998)
Peijun Zhang, Chikashi Toyoshima, Koji Yonekura, N. Michael Green & David L. Stokes



Resources
Atomic Co-ordinates of Ca²⁺ -ATPase in various states...
aligned with the membrane domain and oriented so that the lipid
bilayerruns are parallel to the x-y plane with z=0 at the middle of the bilayer.
Two molecules in the MgF complex are somewhat different: A is presumably closer to E2.
　　　　　E1・２Ca²⁺
　　　　　E1・ATP
　　　　　E1・AlF₄^-・ADP
　　　　　E2・BeF₃^- (-TG)
　　　　　E2・BeF₃^- (+TG)
　　　　　E2・AlF₄^-
　　　　　E2・MgF₄^2- molA
　　　　　E2・MgF₄^2- molB
　　　　　E2（TG）

Secondary structure and mutation information （JPEG, CorelDraw, or TIFF)

Crystals of membrane proteins

Manuals of cryo-TEM method

Simulaion movies...
conformational stability of the Ca²⁺-binding sites in SR Ca²⁺-ATPase studied by molecular dynamics simulatoins. Cyan and red spheres represent Ca²⁺ and water, respectively (J.Am.Chem.Soc.127 (2005) 6160-6151).
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